Composite

Part:BBa_K3766112:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-10-21)


sfGFP-LVAtag expression cassette under the control of pTacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

primer design for Golden gate self assembly

pTacI_F ATAGGTCTCACTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTCCCGCCCATACAAAATAAGGAGC

pTacI_R ATAGGTCTCACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG

Source

PCR amplification on BBa_K2675056 in pSB3T5

References